بررسي اختلاط نانو ذرات سلنيوم بر فعاليت ضد قارچي و ضد باكتريايي تيشو كانديشنر

Background an‎d Objective: Tissue conditioners are commonly used as supportive an‎d adjunctive treatments in dentistry. However, microbial resistance an‎d biofilm formation can compromise their effectiveness. Selenium nanoparticles, due to their potent antimicrobial an‎d antioxidant properties, hold great promise for enhancing the antimicrobial performance of tissue conditioners. This study aimed to investigate the impact of incorporating selenium nanoparticles on the antifungal an‎d antibacterial activity of tissue conditioners. Materials an‎d Methods: This experimental laboratory study prepared tissue conditioner samples with selenium nanoparticles added at weight concentrations of 0.625%, 5%, 10%, an‎d 20%. These samples were tested against three target microorganisms: Can‎dida albicans , Enterococcus faecalis, an‎d Streptococcus mutans. eva‎luations included measurement of inhibition zone diameter, minimum inhibitory concentration (MIC), an‎d minimum bactericidal concentration (MBC). Anti-biofilm effects were also assessed using microtiter plate assays, qualitative tube adherence tests, an‎d scanning electron microscopy. Results: Results demonstrated that selenium nanoparticles at 20% concentration significantly enhanced the antibacterial activity of the tissue conditioner against Enterococcus faecalis an‎d Streptococcus mutans (inhibition zones of 14 mm an‎d 27 mm, respectively; p <‎ 0.05). The MIC an‎d MBC values confirmed effective bacterial growth inhibition at this concentration. Conversely, no significant antifungal effect was observed against Can‎dida albicans , as fungal growth persisted at all tested concentrations. Electron microscopy analyses confirmed reduced biofilm formation an‎d structural microbial changes. Conclusion: Selenium nanoparticles exhibit strong potential to reinforce the antimicrobial properties of tissue conditioners, particularly against bacteria implicated in oral diseases. Nonetheless, to enhance antifungal efficacy, further research involving combinations with other antifungal agents an‎d novel synthesis methods is necessary. Additionally, comprehensive studies on biocompatibility an‎d clinical applications of this composite are essential. Keywords: Selenium nanoparticles, tissue conditioner, antibacterial activity, antifungal activity, biofilm, Can‎dida albicans , Enterococcus faecalis, Streptococcus mutans

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معصومه رستم زاده، صفورا درخشان